By Plamen Demirev, Todd R. Sandrin
In the final sector century, advances in mass spectrometry (MS) were on the leading edge of efforts to map complicated organic structures together with the human metabolome, proteome, and microbiome. All of those advancements have allowed MS to develop into a well-established molecular point know-how for microorganism characterization. MS has validated its significant virtue as a fast, exact, and low in cost strategy for microorganism id, in comparison to traditional phenotypic concepts. within the final a number of years, purposes of MS for microorganism characterization in examine, medical microbiology, counter-bioterrorism, foodstuff protection, and environmental tracking were documented in millions of guides. Regulatory our bodies in Europe, the united states, and somewhere else have authorized MS-based assays for infectious illness diagnostics. As of mid-2015, greater than 3300 advertisement MS structures for microorganism id were deployed all over the world in hospitals and medical labs.
While prior paintings has coated broader techniques in utilizing MS to represent microorganisms on the species point or above, this e-book makes a speciality of strain-level and subtyping purposes. In twelve person chapters, innovators, leaders and practitioners within the box from world wide have contributed to a entire assessment of present and next-generation techniques for MS-based microbial characterization on the subspecies and pressure degrees. Chapters comprise up to date reference lists in addition to web-links to databases, advised software program, and different worthwhile tools.
The emergence of latest, antibiotic-resistant traces of human or animal pathogens is of outstanding difficulty not just to the clinical and clinical groups, yet to most people in addition. advancements of novel MS-based assays for quick identity of traces of antibiotic-resistant microorganisms are reviewed within the e-book to boot. Microbiologists, bioanalytical scientists, infectious disorder experts, scientific laboratory and public future health practitioners in addition to researchers in universities, hospitals, executive labs, and the pharmaceutical and biotechnology industries will locate this ebook to be a well timed and necessary source.
Read or Download Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance PDF
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Additional resources for Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance
2010). A second advantage of the LC-ESI-MS approach is the increased mass accuracy of the measurement, making possible the detection of small mass differences between proteins. These mass differences can be equivalent to single-nucleotide polymorphisms (SNP) mutations or post-translational modifications. Recent work by McFarland and coworkers (2014) best illustrates the implementation of LC(ESI)-MS (intact protein mass) and MS/MS top-down analyses to bacteria differentiation at the strain level.
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